Utility of 16S rDNA Sequencing for Identification of Rare Pathogenic Bacteria
نویسندگان
چکیده
منابع مشابه
Molecular Identification of Rare Clinical Mycobacteria by Application of 16S-23S Spacer Region Sequencing
Objective(s) In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical ...
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BACKGROUND Cases of infective endocarditis (IE) require prompt etiological diagnosis for effective treatment. Molecular methods can aid in rapid and reliable diagnosis of culture-negative IE cases. We evaluated the utility of 16S rDNA PCR and sequencing in determining the causative agents of IE in valve tissues, especially when specimens were obtained after initiation of antimicrobial therapy. ...
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DNA corresponding to 16S rDNA and the 165-23S rDNA intergenic spacer (ITS) from 22 reference strains of acetic acid bacteria, representing the diversity of the family Acetobacteraceae, and 24 indigenous acetic acid bacteria isolated from wine fermentations were analysed by PCR-RFLP. Frateuria aurantia LMG 1558T and Escherichia coli ATCC 11775T were included as outgroups. PCR-amplified products ...
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Aeromonas spp infections are probably the most common bacterial disease diagnosed in cultured warm water fish. In the present study, six strains of Aeromonas spp bacteria were isolated from the gourami (Colisa lalia) by 16S rDNA sequencing analyses that are pathogenic to freshwater fish. Among them, three were under Aeromonas veronii species, two were Aeromonas sp ATCC and one was Aeromonas hyd...
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There have been many attempts to develop sensitive and accurate techniques for the detection and diagnosis of pathogenic bacteria using nucleic acid-based technology. To achieve efficient multiple detection of seven selected food-borne pathogens, we assessed the respective 16S rDNA pathogen specific sequences using an oligonucleotide-based signature array. Strategic optimal design of specific c...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 2016
ISSN: 0887-8013
DOI: 10.1002/jcla.21980